Expression Technologies Inc.

High density bacterial E.coli culture media for plasmid and protein production
DNAGro^TM, ProGro^TM, AutoX^TM, DetoX^TM, InduX^TM, and SecPro^TM media

High density bacterial E.coli culture media

Features and benefits of high density culture media

Applications of high density culture media

More information for high density culture media...>
More information about yield, solubility, stability, and activity...>

Back to top ^    |    Go to bottom _v

High density bacterial E.coli culture medium DNAGro^TM
For plasmid DNA and phage production

For any given plasmid (copy number of the plasmid is determined), there are two ways to increase plasmid yield. The first way is to increase the cell density or the number of cells. The other way is to increase the percentage of cells containing the plasmid. Adding chloramphenicol to the medium may amplify some plasmids but not others. E.coli culture medium DNAGro^TM is proprietarily formulated for plasmid DNA and phage production. Most commonly-used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. Recombinant protein cannot be expressed in this medium because the medium composition inhibits protein induction by IPTG, arabinose, or any sugar-based inductions. Protein induction may be achieved in this medium if heat or salt inducible promoters are used. Ten times or higher cell density may be obtained from this medium when compared to that from LB broth when culture conditions are closely followed. Plasmid or phage DNA yields will increase accordingly if there is sufficient selection.

Click on above product names for literatures of the high density bacterial E.coli culture medium DNAGro^TM

MSDS of the high density bacterial E.coli culture medium DNAGro^TM

Liquid culture medium MSDS
Powder culture medium MSDS

Back to top ^    |    Go to bottom _v

High density bacterial E.coli culture medium ProGro^TM
For recombinant protein production

E.coli culture medium ProGro^TM is proprietarily formulated for recombinant protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. Recombinant proteins can be expressed in this medium regardless whether they are induced by IPTG, sugar, heat or salt. Ten times or higher cell density may be obtained in this medium than that from LB broth when culture conditions are closely followed. Recombinant protein yield will increase accordingly if there is sufficient selection. This culture medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

Click on above product names for literatures of the high density bacterial E.coli culture medium ProGro^TM

MSDS of the high density bacterial E.coli culture medium ProGro^TM

Liquid culture medium MSDS
Powder culture medium MSDS

Back to top ^    |    Go to bottom _v

High density bacterial E.coli culture medium AutoX^TM
For automatic recombinant protein expression without adding IPTG

E.coli culture medium AutoX^TM is proprietarily formulated for recombinant protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. Recombinant proteins can be automatically expressed in this medium if they are induced by IPTG. Induction occurs after cell density reaches OD600 = 10. A specific inducer must be added if the recombinant protein is not induced by IPTG or lactose. Cell density ten times or higher may be obtained in this medium when compared to that from LB broth when culture conditions are closely followed. Recombinant protein yield will increased accordingly if there is sufficient selection. This culture medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

Click on above product names for literatures of the high density bacterial E.coli culture medium AutoX^TM

MSDS of the high density bacterial E.coli culture medium AutoX^TM

Liquid culture medium MSDS
Powder culture medium MSDS

Back to top ^    |    Go to bottom _v

High density bacterial E.coli culture medium DetoX^TM
For toxic recombinant protein production

DetoX^TM E.coli culture medium is proprietarily formulated for toxic protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. This medium is designed for E.coli cell culture and propagation, but not for recombinant protein expression or production. The inhibitors in this medium will prohibit protein expression induced by IPTG, lactose, arabinose, or any other sugars. After cells reach inducible OD (5 to 20 for most cell strains or recombinant proteins), other high density culture media such as InduX^TM or ProGro^TM should be transferred to the cell pellet and induction may be performed in these media instead. Using common bacterial culture medium such as LB will result in lower yield. The medium has no effect on protein expression induced by heat or salt. This culture medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

Click on above product names for literatures of the high density bacterial E.coli culture medium DetoX^TM

MSDS of the high density bacterial E.coli culture medium DetoX^TM

Liquid culture medium MSDS
Powder culture medium MSDS

Back to top ^    |    Go to bottom _v

High density bacterial E.coli culture medium InduX^TM
For toxic recombinant protein production with DetoX^TM medium

InduX^TM E.coli culture medium is proprietarily formulated for recombinant protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this medium. Recombinant proteins can be expressed in this medium regardless whether they are induced by IPTG, any other sugars, heat or salt. Ten times or higher cell density may be obtained in this medium than that from LB broth when culture conditions are closely followed. Recombinant protein yield will increase accordingly if there is sufficient selection. No IPTG is needed if the recombinant protein is induced by IPTG or lactose. A specific inducer must be added at induction if the protein is not induced by IPTG or lactose. This culture medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

Click on above product names for literatures of the high density bacterial E.coli culture medium InduX^TM

MSDS of the high density bacterial E.coli culture medium InduX^TM

Liquid culture medium MSDS
Powder culture medium MSDS

Back to top ^    |    Go to bottom _v

High density bacterial E.coli culture medium SecPro^TM
For secretory recombinant protein production

SecPro^TM E.coli culture medium is proprietarily formulated for secretory protein expression. Most commonly used E.coli strains can reach OD600 = 20 in a shake flask container in this  medium. Recombinant proteins can be expressed in this medium regardless whether they are induced by IPTG, any other sugars, heat or salt. Ten times or higher cell density may be obtained in this medium when compared to that from LB broth when culture conditions are closely followed. Secretory protein expression will increase one hundred times or more. This culture medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein. Some mutant cell strains or strains with toxic proteins do not grow well in this medium. In these cases, these cell strains should be grown in DetoX^TM or ProGro^TM medium first to reach inducible OD. Then change the medium to SecPro^TM for induction.

Click on above product names for literatures of the high density bacterial E.coli culture medium SecPro^TM

MSDS of the high density bacterial E.coli culture medium SecPro^TM

Liquid culture medium MSDS
Powder culture medium MSDS

Back to top ^    |    Go to bottom _v

FAQs of high density bacterial E.coli culture media

1. Why should a high density culture medium be used for a project?
2. How does one select a high density culture medium?
3. Can DNAGro^TM culture medium increase the yield for any plasmid?
4. Can DNAGro^TM culture medium be used for protein expression?
5. Can chloramphenicol be used in DNAGro^TM culture medium?
6. Can ProGro^TM, AutoX^TM, and InduX^TM culture media increase the yield for any recombinant protein?
7. Can DetoX^TM culture medium reduce the toxicity of any protein to the host cells?
8. Why can these high density culture media increase solubility of a recombinant protein?
9. Will these high density culture media increase solubility for any insoluble protein?
10. Can these high density culture medium increase protein stability or activity?
11. What are the typical conditions used for the high density culture media?
12. What are the highest OD600 values from different shake flask containers?
13. What are the OD600 values at normal shaking speed?
14. Can high density culture media be used at low temperatures?
15. What are the differences between glass and plastic flasks? How about different plastics?
16. What are the differences between baffled and regular flasks?
17. What are the differences among conical, round- bottom, and square-bottom tubes?
18. Why can't I reach OD600 = 30 to 50 even though the high density media were shaken at 350 to 450 rpm?
19. Can we use the high density media in deep-well plates? What will be the OD?
20. Why do I sometimes get OD higher than 50?
21. Can all high density culture media reach OD600 = 30 to 50?
22. How do these high density culture media compare to other special media?
23. What are the benefits of using high density culture media?

Examples of using high density culture media

Price list of high density E.coli culture media

Warranty of high density bacterial culture  media

We guarantee our high density bacterial culture media can support E.coli culture over OD₆₀₀ = 20 in a shake flask container. If the aeration conditions are carefully followed, the cell density can reach OD₆₀₀ = 30 to 50. Protein and plasmid yields will increase accordingly. If our products fail to meet such specifications, Exptec will promptly replace the product. Please email us at info@exptec.com for more information.

In addition to medium contents, protein yield, solubility, stability, and activity are also related to other factors. Please click on respective words for more information.

Related products of high density culture media

Commonly used culture media
DNA ladders or DNA markers
Expression vectors
Competent cells for cloning and expression
E.coli cell strains for protein expression

Related literatures of high density culture media

Bacterial culture media
Plasmid DNA yield
Protein yield
Protein solubility
Toxic protein cloning and expression

Protocols of culture media

Plasmid DNA mini-prep
Protein induction analysis
Protein solubility analysis
Protein prep with ProGro^TM medium

Notes to international customers

1. All liquid media will be shipped to international customers only when the shipping cost is prepaid. By providing us with the account number of the preferred carrier, we can charge shipping costs directly to the customer's account. The shipping charges posted on our web site serve as packing and handling fees for international purchases.
2. Since liquid media cannot be exposed to extreme temperatures, we will use express delivery if liquid media are ordered.
3. Our customers may choose their preferred shipping methods. In these cases, we CANNOT guarantee the delivery conditions of the liquid media.
4. It is our customers' responsibility to pay all the duties, taxes, and fees associated with importation and The Customs.
5. It is important to get The Customs clearance before purchasing our products. We will not refund the purchase if the products are returned by The Customs.
6. MSDS for each medium may be downloaded from this page under each product. MSDS may be needed for The Customs clearance.

Please email us at info@exptec.com for more information.

Back to top ^

[ Home ] [ Up ]
Copyright © 2003 Expression Technologies Inc.