Expression Technologies Inc.

tRNA

Different organisms use different set of genetic codons. Some heavily used codons in mammal may be rarely used in E.coli. Codon starvation results in no expression or low yield of some mammalian proteins in E.coli. tRNA Plus strains are designed to solve these problems and increase protein yield.

tRNA plus strains over-express one or more tRNA genes from E.coli. This will overcome codon starvation problems. We developed two sets of tRNA plus strains. Each set of tRNA plus strain contain one or more tRNA genes respectively. They are named PlustRNA-gene-name. tRNA-gene-name is commonly used name for tRNA genes. For example, R4 stands for tRNA gene Arg4. It is also called dnaY gene or argU tRNA gene. PlusR4 strain over expresses tRNA gene Arg4. Its tRNA contains anticodon UCU will recognize codons AGA and AGG.

Other factors also affect protein yield. Please click here for more information on protein yield.

tRNA plus strains overexpress tRNA genes:

Strain Name	Cat#	tRNA Gene	Anticodon	Rare Codon Recognition	DNA Codon	Price (US$)
PlusI2a^TM	10011	Ile2(ileX)	CAU	AUA	ATA	150
PlusL3MQ12a^TM	10012	Leu3(leuW), Metm(metT), Gln1(glnU), Gln2(glnV)	UAG, AUT, UUG, CUG,	CUA	CTA, ATG, CAA, CAG	150
PlusP2a^TM	10013	Pro2(proL, proW)	GGG	CCC,CCU	CCC, CCT	150
PlusR2S3a^TM	10014	Arg2(argV), Ser3(serV)	ACG, GCU	CGA, AGU	CGA, AGC, AGT	150
PlusR3HL1P3a^TM	10015	Arg3(argT), His(hisR), Leu(leuT), Pro3(proU)	CCG, GUG, CAG, UGG	CGG, CCA, CCU	CGG, CAC, CAT, CTG, CCA, CCT	150
PlusR4a^TM	10016	Arg4(dnaY, argU)	UCU	AGA, AGG	AGA, AGG	150
PlusR5a^TM	10017	Arg5(argW)	CCU	AGG	AGG	150
PlusI2b^TM	10021	Ile2(ileX)	CAU	AUA	ATA	150
PlusL3MQ12b^TM	10022	Leu3(leuW), Metm(metT), Gln1(glnU), Gln2(glnV)	UAG, AUT, UUG, CUG,	CUA	CTA, ATG, CAA, CAG	150
PlusP2b^TM	10023	Pro2(proL, proW)	GGG	CCC,CCT	CCC, CCT	150
PlusR2S3b^TM	10024	Arg2(argV), Ser3(serV)	ACG, GCU	CGA	CGA, AGC, AGT	150
PlusR3HL1P3b^TM	10025	Arg3(argT), His(hisR), Leu(leuT), Pro3(proU)	CCG, GUG, CAG, UGG	CGG, CCA, CCU	CGG, CAC, CAT, CTG, CCA, CCT	150
PlusR4b^TM	10026	Arg4(dnaY, argU)	UCU	AGA, AGG	AGA AGG	150
PlusR5b^TM	10027	Arg5(argW)	CCU	AGG	AGG	150

All the strain names ending with "a" are used in the expression with E.coli RNA polymerase and strain names ending with "b" are used in the expression with T7 RNA polymerase. All of the cell strains with ^TM superscripts are trademarks of Expression Technologies Inc. They are all patent-pending. These cell strains are for non-commercial research use only. Please contact us for any other uses.

Warranty

These cell strains are guaranteed to express at least three times or more than the endogenous expression level of specified gene(s). Please email us at info@exptec.com for more information.