Expression Technologies Inc.

Other Detox

Our DetoxC^TM, DetoxE^TM, and DetoxET7^TM strains are not compatible with plasmids with pACYC replication origins. In addition, these cell strains are chloramphenicol resistant. Plasmids with chloramphenicol resistance selection markers cannot be selected in these cell strains. Here we have other detoxification strains that are compatible with different plasmids, with different antibiotic resistances, and express different levels of lacI repressor. More on toxic protein cloning and expression...

Strain Name	Cat Number	Compatible Plasmid	Resistance	LacI level	Price(US$)
DetoxAAa^TM	11101	ColE1	Amp	Low	500
DetoxAAb^TM	11102	ColE1	Amp	Low	500
DetoxAAc^TM	11103	ColE1	Amp	Low	500
DetoxACa^TM	11111	ColE1	Chl	Low	500
DetoxACb^TM	11112	ColE1	Chl	Low	500
DetoxACc^TM	11113	ColE1	Chl	Low	500
DetoxAKa^TM	11121	ColE1	Kan	Low	500
DetoxAKb^TM	11122	ColE1	Kan	Low	500
DetoxAKc^TM	11123	ColE1	Kan	Low	500
DetoxATa^TM	11131	ColE1	Tet	Low	500
DetoxATb^TM	11132	ColE1	Tet	Low	500
DetoxATc^TM	11133	ColE1	Tet	Low	500
DetoxBAa^TM	11201	pACYC	Amp	Medium	500
DetoxBAb^TM	11202	pACYC	Amp	Medium	500
DetoxBAc^TM	11203	pACYC	Amp	Medium	500
DetoxBCa^TM	11211	pACYC	Chl	Medium	500
DetoxBCb^TM	11212	pACYC	Chl	Medium	500
DetoxBCc^TM	11213	pACYC	Chl	Medium	500
DetoxBKa^TM	11221	pACYC	Kan	Medium	500
DetoxBKb^TM	11222	pACYC	Kan	Medium	500
DetoxBKc^TM	11223	pACYC	Kan	Medium	500
DetoxBTa^TM	11231	pACYC	Tet	Medium	500
DetoxBTb^TM	11232	pACYC	Tet	Medium	500
DetoxBTc^TM	11233	pACYC	Tet	Medium	500
DetoxCAa^TM	11301	pACYC	Amp	High	500
DetoxCAb^TM	11302	pACYC	Amp	High	500
DetoxCAc^TM	11303	pACYC	Amp	High	500
DetoxCCa^TM	11311	pACYC	Chl	High	500
DetoxCCb^TM	11312	pACYC	Chl	High	500
DetoxCCc^TM	11313	pACYC	Chl	High	500
DetoxCKa^TM	11321	pACYC	Kan	High	500
DetoxCKb^TM	11322	pACYC	Kan	High	500
DetoxCKc^TM	11323	pACYC	Kan	High	500
DetoxCTa^TM	11331	pACYC	Tet	High	500
DetoxCTb^TM	11332	pACYC	Tet	High	500
DetoxCTc^TM	11333	pACYC	Tet	High	500
DetoxDAa^TM	11401	ColE1	Amp	High	500
DetoxDAb^TM	11402	ColE1	Amp	High	500
DetoxDAc^TM	11403	ColE1	Amp	High	500
DetoxDKa^TM	11421	ColE1	Kan	Medium	500
DetoxDKb^TM	11422	ColE1	Kan	Medium	500
DetoxDKc^TM	11423	ColE1	Kan	Medium	500
DetoxDTa^TM	11431	ColE1	Tet	High	500
DetoxDTb^TM	11432	ColE1	Tet	High	500
DetoxDTc^TM	11433	ColE1	Tet	High	500

Strain names ending with "a", "b", and "c" are used for cloning and expression with E.coli RNA polymerase, expression only with E.coli RNA polymerase and expression with T7 RNA polymerase respectively. These strains express comparable level of lacI. All of our detoxification strains express lacI consecutively. Cell strains with low, medium, and high level of lacI express about 10, 20 to 50, and 100 times more lacI repressor than indigenous level respectively. DetoxC^TM, DetoxE^TM, and DetoxET7^TM strains express medium level of lacI repressor. When a recombinant protein has low toxicity and high yield is desired, cell strains with lower level of lacI should be used. Our special media are sufficient to express most low toxic proteins. For extremely toxic protein, high lacI expressing cell strains, different vectors and protocol may be used. Plasmids generated from pBR322 or pUC19 all have ColE1 replication origins. pACYC plasmids are generated from pACYC177 and pACYC184 plasmids. All above cell strains are compatible with plasmids generated from replication origins other than ColE1 and pACYC.

All of the cell strains with ^TM superscripts are trademarks of Expression Technologies Inc. They are all proprietary products. These cell strains are for non-commercial research use only and they cannot be distributed out of the purchasing lab. Please contact us for any other uses. Please email us at info@exptec.com for more information.