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E.coli Growth Media
 

Bacterial E.coli Growth Media
LB, SOB, SOC, 2x YT, TB (Terrific Broth), SB (Super Broth), and high density growth media

Product list of bacterial E.coli growth media

Commonly used bacterial E.coli growth media

bullet LB (Luria Bertani) Miller broth (1%NaCl): 1% peptone, 0.5% yeast extract, and 1% NaCl
bullet LB (Luria Bertani) Lennox Broth (0.5% NaCl): 1% peptone, 0.5% yeast extract, and 0.5% NaCl
bullet SOB medium (Super Optimal Broth): 2% peptone, 0.5% Yeast extract, 10mM NaCl, 2.5mM KCl, 10mM MgCl2 , 10mM MgSO4
bullet SOC medium (Super Optimal broth with Catabolic repressor): SOB + 20mM glucose
bullet 2x YT broth (2x Yeast extract and Tryptone): 1.6% peptone, 1% yeast extract, and 0.5% NaCl
bullet TB (Terrific Broth) medium: 1.2% peptone, 2.4% yeast extract, 72 mM K2HPO4, 17 mM KH2PO4 and 0.4% glycerol
bullet SB (Super Broth) medium: 3.2% peptone, 2% yeast extract, and 0.5% NaCl

High density bacterial E.coli growth media

bullet DNAGroTM medium: Support E.coli growth to OD600 = 30 - 50. Increase plasmid DNA yield 10 times over LB
bullet ProGroTM medium: Support E.coli growth to OD600 = 30 - 50. Increase protein yield 10 times over LB
bullet AutoXTM medium: Support E.coli growth to OD600 = 30 - 50. Increase protein yield 10 times over LB without adding IPTG
bullet DetoXTM medium: Reduce protein toxicity and support E.coli growth to OD600 = 30
bullet InduXTM medium: Support E.coli growth to OD600 = 30. Increase protein yield, solubility, stability and activity
bullet SecProTM medium: Increase secretary protein expression over 100 times than LB or TB

Applications of common media >... | special media >...

Commonly used bacterial E.coli growth media
LB, SOB, SOC, 2x YT, Terrific Broth (TB), and Super Broth (SB)

Applications of common bacterial growth media

bulletE.coli growth and propagation
bulletPlasmid DNA prep and production
bulletProtein expression and production
bulletCompetent cell preparation
bulletTransformation
bulletPhage production
 
Medium Name

Applications

LB Miller Broth

E.coli growth and propagation; plasmid DNA and protein production

LB Lennox Broth E.coli growth and propagation; plasmid DNA and protein production
SOB Medium

Prepare high efficiency competent cells; plasmid DNA and protein production

SOC Medium Plasmid transformation and growth of competent cells
2x YT Medium Phage DNA production
Terrific Broth (TB) High yield protein and plasmid DNA production
Super Broth (SB) High yield plasmid DNA and protein production

More information of common bacterial growth media...>

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Bacterial E.coli growth media: LB Miller broth/LB Lennox broth

LB broth is the most commonly used medium in molecular biology for E.coli cell culture. Easy to make, fast growth rate of most E.coli strains, readily available and simple compositions contribute the popularity of LB broth. LB broth contains the enzymatic digestion product of casein commonly known as peptone (some vendors term it Tryptone), yeast extract, and sodium chloride. Peptone is rich in amino acids and peptides. Its amino acid and peptide compositions reflect those of casein. In addition to amino acids and peptides, yeast extract also contains nucleic acids, lipids and other nutrients which are needed for bacterial growth. LB can support E.coli growth OD600 2 to 3 under normal shaking incubation condition (250 rpm). The LB Miller Broth and LB Lennox Broth contain higher and lower sodium chloride levels respectively. There are minimal differences between the two formulations in most molecular biology studies with commonly used E.coli strains. Different bacteria strains may require different salt concentrations. The low salt formula is more often used in salt-sensitive antibiotic selections.

Our LB broth is best priced in the industry at 12 bottle packages.

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High salt LB broth/LB medium: LB Miller Broth
1% peptone, 0.5% yeast extract, and 1% NaCl

High salt LB broth/LB medium

Catalog # Price (US$)

Add to Cart l

Sterilized liquid LB Miller Broth
500 ml/bottle, 1 bottle
40001 12
Sterilized liquid LB Miller Broth
1 liter/bottle, 1 bottle
40002 20
Sterilized liquid LB Miller Broth
500 ml/bottle, 12 bottles
40003 96
Sterilized liquid LB Miller Broth
1 liter/bottle, 12 bottles
40004 180

Product literatures of bacterial E.coli growth medium high salt LB Broth/LB medium

Technical sheet | MSDS

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Low salt LB broth/LB medium: LB Lennox Broth
1% peptone, 0.5% yeast extract, and 0.5% NaCl

Low salt LB broth/LB medium

Catalog # Price (US$)

Add to Cart l

Sterilized liquid LB Lennox Broth
500 ml/bottle,1 bottle
40011 12
Sterilized liquid LB Lennox Broth
1 liter/bottle, 1 bottle
40012 20
Sterilized liquid LB Lennox Broth
500 ml/bottle, 12 bottles
40013 96
Sterilized liquid LB Lennox Broth
1 liter/bottle, 12 bottles
40014 180

Product literatures of bacterial E.coli growth medium low salt LB Broth/LB medium

Technical sheet | MSDS

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Bacterial E.coli growth medium SOB
2% peptone, 0.5% Yeast extract, 10mM NaCl, 2.5mM KCl, 10mM MgCl2 , 10mM MgSO4

SOB is another commonly used rich media. It has twice as much peptone compared to LB. It is richer in amino acids and peptides. In addition, it contains magnesium Mg2+ which is required for high density cell growth. SOB can support E.coli growth to OD600 3 to 5 under normal shake flask conditions (250 rpm). It can support cell density to OD600 10 to 15 with increased aeration (350 rpm). E.coli cells growing in SOB will have higher transformation efficiency than those in a LB medium. SOB is commonly used to make high efficiency competent cells.

SOB medium

Catalog #

Price (US$)

Add to Cart l

Sterilized liquid SOB medium
500 ml/bottle, 1 bottle
40031 30
Sterilized liquid SOB medium
1 liter/bottle, 1 bottle
40032 50

Product literatures of bacterial E.coli growth medium SOB

Technical sheet | MSDS

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Bacterial E.coli growth medium SOC
2% peptone, 0.5% Yeast extract, 10mM NaCl, 2.5mM KCl, 10mM MgCl2 , 10mM MgSO4, 20 mM Glucose

SOC is the same as SOB except it contains 20 mM glucose. Glucose serves as a catabolic repressor in SOC medium. E.coli cells prefer to use glucose as a carbon or energy source. In the presence of glucose, cellular machineries that use other sugars will be repressed. At a concentration of 20 mM or 0.36%, it provides sufficient transcription repression for lacI repressed promoters at low cell density. Expression of recombinant proteins that may adversely affect host cell physiology is repressed. As a result, transformation reactions using SOC to grow the cells for one hour will have higher efficiencies than those using LB. SOC is commonly used in incubation after heat shock in the transformation reaction. When SOC is not available, adding 20 mM glucose to LB or any other commonly used rich media will result in similar transformation efficiency as that can be obtained from using SOC.

SOC medium

Catalog #

Price (US$)

Add to Cart l

Sterilized liquid SOC Medium
40 ml/tube, 1 tube
40021 12
Sterilized liquid SOC Medium
40 ml/tube, 2 tubes
40022 20

Product literatures of bacterial E.coli growth medium SOC

Technical sheet | MSDS

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Bacterial E.coli growth medium 2x YT broth
1.6% peptone, 1% yeast extract, and 0.5% NaCl

2x YT broth is named in comparing its nutrition content to that of LB broth. It does have 2x as much yeast extract compared to LB broth, but it only has 60% (not exactly 2x) more peptone. Basically 2x YT is a richer medium than LB and can support higher cell density and a longer growth period for E.coli. 2x YT is originally formulated for the growth and maintenance of E.coli and its fibrous bacteriophage such as the M13 phage allows relatively large quantity phage production without exhausting the host.

2x YT broth or medium

Catalog #

Price (US$)

Add to Cart l

Sterilized liquid 2x YT Medium
500 ml/bottle, 1 bottle
40051 12
Sterilized liquid 2x YT Medium
1 liter/bottle, 1 bottle
40052 20
Sterilized liquid 2x YT Medium
500 ml/bottle, 12 bottles
40053 115
Sterilized liquid 2x YT Medium
1 liter/bottle, 12 bottles
40054 192

Product literatures of bacterial E.coli growth medium 2xYT broth or medium

Technical sheet | MSDS

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Bacterial E.coli growth medium TB or Terrific Broth
1.2% peptone, 2.4% yeast extract, 72 mM K2HPO4, 17 mM KH2PO4, and 0.4% glycerol

TB or Terrific broth is a phosphate buffered rich medium. In addition to 20% more peptone and 380% more yeast extract than LB broth, TB also has an added 0.4% glycerol as an extra carbon source. All these nutrients in TB can support E.coli growth to OD600 5 to 8 under normal shaking incubation conditions. TB is commonly used for protein expression and plasmid production in a laboratory scale.

Terrific broth or TB

Catalog #

Price (US$)

Add to Cart l

Sterilized liquid TB Medium
500 ml/bottle, 1 bottle
40041 18
Sterilized liquid TB Medium
1 liter/bottle, 1 bottle
40042 28
Sterilized liquid TB Medium
500 ml/bottle, 12 bottles
40043 172
Sterilized liquid TB Medium
1 liter/bottle, 12 bottles
40044 268

Product literatures of bacterial E.coli growth medium TB or Terrific Broth

Technical sheet | MSDS

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Bacterial E.coli growth medium SB or Super Broth
3.2% peptone, 2% yeast extract, and 0.5% NaCl

SB or Super Broth has 220% more peptone and 300% more yeast extract than LB. Therefore SB is supper rich in peptone and yeast extract. We estimated that the major nutrients in peptone and yeast extract are still in excess well after cells reach saturation while trace nutrients are depleted in the same culture. SB is used for plasmid or protein production.

SB or Super Broth

Catalog # Price (US$)

Add to Cart l

Sterilized liquid SB Medium
500 ml/bottle, 1 bottle
40061 18
Sterilized liquid SB Medium
1 liter/bottle, 1 bottle
40062 28
Sterilized liquid SB Medium
500 ml/bottle, 12 bottles
40063 172
Sterilized liquid SB Medium
1 liter/bottle, 12 bottles
40064 268

Product literatures of bacterial E.coli growth medium SB or Super Broth

Technical sheet | MSDS

FAQs of commonly used bacterial E.coli growth media

  1. How do you  select a commonly used growth medium for a research project?
  2. Why is LB broth the most used medium in many academic labs?
  3. What is the difference between LB Miller and LB Lennox broths?
  4. Will magnesium (Mg2+) increase the cell densities for commonly used bacterial E.coli growth media?
  5. Will agitation increase the cell density for commonly used bacterial E.coli growth media?
  6. What is the best commonly used E.coli growth medium for plasmid production?
  7. What is the best commonly used E.coli growth medium for protein production?
  8. What is the best commonly used E.coli growth medium for phage production?
  9. What is the best commonly used E.coli growth medium for making competent cells?
  10. What is the highest cell density that may be obtained from commonly used bacterial E.coli growth media?

Price list of commonly used bacterial E.coli growth media

Common Medium Cat # Price (US$) Add to Cart   
Sterilized liquid LB Miller broth
500 ml/bottle, 1 bottle
40001 12
Sterilized liquid LB Miller broth
1 liter/bottle, 1 bottle
40002 20
Sterilized liquid LB Miller broth
500 ml/bottle, 12 bottles
40003 96
Sterilized liquid LB Miller broth
1 liter/bottle, 12 bottles
40004 180
Sterilized liquid LB Lennox broth
500 ml/bottle, 1 bottle
40011 12
Sterilized liquid LB Lennox broth
1 liter/bottle, 1 bottle
40012 20
Sterilized liquid LB Lennox broth
500 ml/bottle, 12 bottles
40013 96
Sterilized liquid LB Lennox broth
1 liter/bottle, 12 bottles
40014 180
Sterilized liquid SOC Medium
40 ml/tube, 1 tube
40021 12
Sterilized liquid SOC Medium
40 ml/tube, 2 tubes
40022 20
Sterilized liquid SOB Medium
500 ml/bottle, 1 bottle
40031 30
Sterilized liquid SOB Medium
1 liter/bottle, 1 bottle
40032 50
Sterilized liquid TB Medium
500 ml/bottle, 1 bottle
40041 18
Sterilized liquid TB Medium
1 liter/bottle, 1 bottle
40042 28
Sterilized liquid TB Medium
500 ml/bottle, 12 bottles
40043 172
Sterilized liquid TB Medium
1 liter/bottle, 12 bottles
40044 268
Sterilized liquid 2x YT broth
500 ml/bottle, 1 bottle
40051 12
Sterilized liquid 2x YT broth
1 liter/bottle, 1 bottle
40052 20
Sterilized liquid 2x YT broth
500 ml/bottle, 12 bottles
40053 115
Sterilized liquid 2x YT broth
1 liter/bottle, 12 bottles
40054 192
Sterilized liquid SB Medium
500 ml/bottle, 1 bottle
40061 18
Sterilized liquid SB Medium
1 liter/bottle, 1 bottle
40062 28
Sterilized liquid SB Medium
500 ml/bottle, 12 bottles
40063 172
Sterilized liquid SB Medium
1 liter/bottle, 12 bottles
40064 268

Warranty of common bacterial growth media

We guarantee the composition and sterilization of our common media. If our products fail to meet such specifications, Exptec will promptly replace the product. In addition, we also guarantee the prices of most our common media. Please email us at info@exptec.com for more information.

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High density bacterial E.coli growth media
DNAGroTM, ProGroTM, AutoXTM, DetoXTM, InduXTM, and SecProTM media

Features and benefits of high density growth media

bulletGrows E.coli cell density to OD600 = 30 to 50 in a shake flask (compared to OD600=2 to 3 in LB) which is comparable to that may be obtained from a regular fed-batch fermentation
bulletIncreases protein or plasmid DNA yield 10 times or higher
bulletIncrease secretory protein expression 100 times or higher
bulletpH is maintained close to optimal (pH 7) for E.coli growth up to OD 600 = 30
bulletContains trace metals, minerals, and vitamins, some of which are needed for E.coli growth and others which of may serve as prosthetic groups, co-factors or ligands for expressed recombinant proteins. These special media increase yield, solubility, stability, and activity of recombinant proteins

Applications of high density growth media

bulletHigh yield plasmid production, 10 times over LB
bulletHigh yield phage production, 10 times over LB
bulletHigh yield protein production, 10 times over LB
bulletHigh yield secretory expression, 100 times over LB or TB
bulletToxic protein expression
bulletIncrease protein solubility
bulletIncrease protein stability
bulletIncrease protein activity

Medium Name

Applications
DNAGroTM growth medium Increases plasmid and phage DNA yields 10 times over LB
ProGroTM growth  medium Increases protein yield, solubility, stability and activity
AutoXTM growth medium Increases protein yield, solubility, stability and activity; automatic protein expression  without adding IPTG
DetoXTM growth medium Reduces protein toxicity and support E.coli growth to OD600 = 30
InduXTM growth medium Increases protein yield, solubility, stability and activity
SecProTM growth medium Increases secretary protein expression over 100 times than LB or TB

More information for high density growth media...>
More information about yield, solubility, stability, and activity...>

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High density bacterial E.coli growth medium DNAGroTM
For plasmid DNA and phage production

For any given plasmid (copy number of the plasmid is determined), there are two ways to increase plasmid yield. The first way is to increase the cell density or the number of cells. The other way is to increase the percentage of cells containing the plasmid. Adding chloramphenicol to the medium may amplify some plasmids but not others. E.coli growth medium DNAGroTM is proprietarily formulated for plasmid DNA and phage production. Most commonly-used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. Recombinant protein cannot be expressed in this medium because the medium composition inhibits protein induction by IPTG, arabinose, or any sugar-based inductions. Protein induction may be achieved in this medium if heat or salt inducible promoters are used. Ten times or higher cell density may be obtained from this medium when compared to that from LB broth when growth conditions are closely followed. Plasmid or phage DNA yields will increase accordingly if there is sufficient selection.

High density growth medium DNAGroTM

Catalog # Price (US$) Add to Cart   
DNAGroTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40101 40
DNAGroTM liquid bacterial growth Medium
500 ml/bottle, 12 bottles
40102 384
DNAGroTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40103 78
DNAGroTM liquid bacterial growth Medium
1 liter/bottle, 12 bottles
40104 748
DNAGroTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40105 70
DNAGroTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40106 560

Click on above product names for literatures of the high density bacterial E.coli growth medium DNAGroTM

MSDS of the high density bacterial E.coli growth medium DNAGroTM

Liquid growth medium MSDS
Powder growth medium MSDS

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High density bacterial E.coli growth medium ProGroTM
For recombinant protein production

E.coli growth medium ProGroTM is proprietarily formulated for recombinant protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. Recombinant proteins can be expressed in this medium regardless whether they are induced by IPTG, sugar, heat or salt. Ten times or higher cell density may be obtained in this medium than that from LB broth when growth conditions are closely followed. Recombinant protein yield will increase accordingly if there is sufficient selection. This growth medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

High density growth medium DNAGroTM

Catalog # Price (US$) Add to Cart   
ProGroTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40111 40
ProGroTM liquid bacterial growth Medium
500 ml/bottle, 12 bottles
40112 384
ProGroTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40113 78
ProGroTM liquid bacterial growth Medium
1 liter/bottle, 12bottles
40114 748
ProGroTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40115 70
ProGroTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40116 560

Click on above product names for literatures of the high density bacterial E.coli growth medium ProGroTM

MSDS of the high density bacterial E.coli growth medium ProGroTM

Liquid growth medium MSDS
Powder growth medium MSDS

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High density bacterial E.coli growth medium AutoXTM
For automatic recombinant protein expression without adding IPTG

E.coli growth medium AutoXTM is proprietarily formulated for recombinant protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. Recombinant proteins can be automatically expressed in this medium if they are induced by IPTG. Induction occurs after cell density reaches OD600 = 10. A specific inducer must be added if the recombinant protein is not induced by IPTG or lactose. Cell density ten times or higher may be obtained in this medium when compared to that from LB broth when growth conditions are closely followed. Recombinant protein yield will increased accordingly if there is sufficient selection. This growth medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

High density growth medium DNAGroTM

Catalog # Price (US$) Add to Cart   
AutoXTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40121 40
AutoXTM liquid bacterial growth Medium
500 ml/bottle, 12 bottles
40122 384
AutoXTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40123 78
AutoXTM liquid bacterial growth Medium
1 liter/bottle, 12 bottles
40124 748
AutoXTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40125 70
AutoXTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40126 560

Click on above product names for literatures of the high density bacterial E.coli growth medium AutoXTM

MSDS of the high density bacterial E.coli growth medium AutoXTM

Liquid growth medium MSDS
Powder growth medium MSDS

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High density bacterial E.coli growth medium DetoXTM
For toxic recombinant protein production

DetoXTM E.coli growth medium is proprietarily formulated for toxic protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. This medium is designed for E.coli cell growth and propagation, but not for recombinant protein expression or production. The inhibitors in this medium will prohibit protein expression induced by IPTG, lactose, arabinose, or any other sugars. After cells reach inducible OD (5 to 20 for most cell strains or recombinant proteins), other high density growth media such as InduXTM or ProGroTM should be transferred to the cell pellet and induction may be performed in these media instead. Using common bacterial growth medium such as LB will result in lower yield. The medium has no effect on protein expression induced by heat or salt. This growth medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

High density growth medium DNAGroTM

Catalog # Price (US$) Add to Cart   
DetoXTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40131 40
DetoXTM liquid bacterial growth Medium
500 ml/bottle, 12 bottles
40132 384
DetoXTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40133 78
DetoXTM liquid bacterial growth Medium
1 liter/bottle, 12 bottles
40134 748
DetoXTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40135 70
DetoXTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40136 560

Click on above product names for literatures of the high density bacterial E.coli growth medium DetoXTM

MSDS of the high density bacterial E.coli growth medium DetoXTM

Liquid growth medium MSDS
Powder growth medium MSDS

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High density bacterial E.coli growth medium InduXTM
For toxic recombinant protein production with DetoXTM medium

InduXTM E.coli growth medium is proprietarily formulated for recombinant protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this medium. Recombinant proteins can be expressed in this medium regardless whether they are induced by IPTG, any other sugars, heat or salt. Ten times or higher cell density may be obtained in this medium than that from LB broth when growth conditions are closely followed. Recombinant protein yield will increase accordingly if there is sufficient selection. No IPTG is needed if the recombinant protein is induced by IPTG or lactose. A specific inducer must be added at induction if the protein is not induced by IPTG or lactose. This growth medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

High density growth medium DNAGroTM

Catalog # Price (US$) Add to Cart   
InduXTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40141 40
InduXTM liquid bacterial growth Medium
500 ml/bottle, 12 bottles
40142 384
InduXTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40143 78
InduXTM liquid bacterial growth Medium
1 liter/bottle, 12 bottles
40144 748
InduXTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40145 70
InduXTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40146 560

Click on above product names for literatures of the high density bacterial E.coli growth medium InduXTM

MSDS of the high density bacterial E.coli growth medium InduXTM

Liquid growth medium MSDS
Powder growth medium MSDS

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High density bacterial E.coli growth medium SecProTM
For secretory recombinant protein production

SecProTM E.coli growth medium is proprietarily formulated for secretory protein expression. Most commonly used E.coli strains can reach OD600 = 20 in a shake flask container in this  medium. Recombinant proteins can be expressed in this medium regardless whether they are induced by IPTG, any other sugars, heat or salt. Ten times or higher cell density may be obtained in this medium when compared to that from LB broth when growth conditions are closely followed. Secretory protein expression will increase one hundred times or more. This growth medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein. Some mutant cell strains or strains with toxic proteins do not grow well in this medium. In these cases, these cell strains should be grown in DetoXTM or ProGroTM medium first to reach inducible OD. Then change the medium to SecProTM for induction.

High density growth medium DNAGroTM

Catalog # Price (US$) Add to Cart   
SecProTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40161 50
SecProTM liquid bacterial growth Medium
500 ml/bottle, 12 bottles
40162 480
SecProTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40163 96
SecProTM liquid bacterial growth Medium
1 liter/bottle, 12 bottles
40164 920
SecProTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40165 80
SecProTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40166 640

Click on above product names for literatures of the high density bacterial E.coli growth medium SecProTM

MSDS of the high density bacterial E.coli growth medium SecProTM

Liquid growth medium MSDS
Powder growth medium MSDS

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FAQs of high density bacterial E.coli growth media

  1. Why should a high density growth medium be used for a project?
  2. How does one select a high density growth medium?
  3. Can DNAGroTM growth medium increase the yield for any plasmid?
  4. Can DNAGroTM growth medium be used for protein expression?
  5. Can chloramphenicol be used in DNAGroTM growth medium?
  6. Can ProGroTM, AutoXTM, and InduXTM growth media increase the yield for any recombinant protein?
  7. Can DetoXTM growth medium reduce the toxicity of any protein to the host cells?
  8. Why can these high density growth media increase solubility of a recombinant protein?
  9. Will these high density growth media increase solubility for any insoluble protein?
  10. Can these high density growth medium increase protein stability or activity?
  11. What are the typical conditions used for the high density growth media?
  12. What are the highest OD600 values from different shake flask containers?
  13. What are the OD600 values at normal shaking speed?
  14. Can high density growth media be used at low temperatures?
  15. What are the differences between glass and plastic flasks? How about different plastics?
  16. What are the differences between baffled and regular flasks?
  17. What are the differences among conical, round- bottom, and square-bottom tubes?
  18. Why can't I reach OD600 = 30 to 50 even though the high density media were shaken at 350 to 450 rpm?
  19. Can we use the high density media in deep-well plates? What will be the OD?
  20. Why do I sometimes get OD higher than 50?
  21. Can all high density growth media reach OD600 = 30 to 50?
  22. How do these high density growth media compare to other special media?
  23. What are the benefits of using high density growth media?

Examples of using high density growth media

bullet InduXTM increases protein yield.
bullet ProGroTM enhance protein solubility and activity.
bullet AutoXTM improve protein yield and stability.

Price list of high density E.coli growth media

High Density Growth Medium

Cat # Price (US$) Add to Cart   
DNAGroTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40101 40
DNAGroTM liquid bacterial growth Medium
500 ml/bottle, 12 bottles
40102 384
DNAGroTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40103 78
DNAGroTM liquid bacterial growth Medium
1 liter/bottle, 12 bottles
40104 748
DNAGroTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40105 70
DNAGroTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40106 560
ProGroTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40111 40
ProGroTM liquid bacterial growth Medium
500 ml/bottle, 12 bottles
40112 384
ProGroTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40113 78
ProGroTM liquid bacterial growth Medium
1 liter/bottle, 12 bottles
40114 748
ProGroTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40115 70
ProGroTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40116 560
AutoXTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40121 40
AutoXTM liquid bacterial growth Medium
500 ml/bottle, 12 bottles
40122 384
AutoXTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40123 78
AutoXTM liquid bacterial growth Medium
1 liter/bottle, 12 bottles
40124 748
AutoXTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40125 70
AutoXTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40126 560
DetoXTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40131 40
DetoXTM liquid bacterial growth Medium
500 ml/bottle, 10 bottles
40132 384
DetoXTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40133 78
DetoXTM liquid bacterial growth Medium
1 liter/bottle, 12 bottles
40134 748
DetoXTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40135 70
DetoXTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40136 560
InduXTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40141 40
InduXTM liquid bacterial growth Medium
500 ml/bottle, 12 bottles
40142 384
InduXTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40143 78
InduXTM liquid bacterial growth Medium
1 liter/bottle, 12 bottles
40144 748
InduXTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40145 70
InduXTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40146 560
SecProTM liquid bacterial growth Medium
500 ml/bottle, 1 bottle
40161 50
SecProTM liquid bacterial growth Medium
500 ml/bottle, 12 bottles
40162 480
SecProTM liquid bacterial growth Medium
1 liter/bottle, 1 bottle
40163 96
SecProTM liquid bacterial growth Medium
1 liter/bottle, 12 bottles
40164 920
SecProTM powdered bacterial growth Medium
powder for 1 liter/pouch, 1 pouch
40165 80
SecProTM powdered bacterial growth Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
 40166 640

Warranty of high density bacterial growth  media

We guarantee our high density bacterial growth media can support E.coli growth over OD600 = 20 in a shake flask container. If the aeration conditions are carefully followed, the cell density can reach OD600 = 30 to 50. Protein and plasmid yields will increase accordingly. If our products fail to meet such specifications, Exptec will promptly replace the product. Please email us at info@exptec.com for more information.

In addition to medium contents, protein yield, solubility, stability, and activity are also related to other factors. Please click on respective words for more information.

Related products of growth media

DNA ladders or DNA markers
Expression vectors
Competent cells for cloning and expression
E.coli cell strains for protein expression

Related literatures of growth media

Bacterial growth media
Plasmid DNA yield
Protein yield
Protein solubility
Toxic protein cloning and expression

Protocols of growth media

Plasmid DNA mini-prep
Protein induction analysis
Protein solubility analysis
Protein prep with ProGroTM medium

Notes to international customers

  1. All liquid media will be shipped to international customers only when the shipping cost is prepaid. By providing us with the account number of the preferred carrier, we can charge shipping costs directly to the customer's account. The shipping charges posted on our web site serve as packing and handling fees for international purchases.
  2. Since liquid media cannot be exposed to extreme temperatures, we will use express delivery if liquid media are ordered.
  3. Our customers may choose their preferred shipping methods. In these cases, we CANNOT guarantee the delivery conditions of the liquid media.
  4. It is our customers' responsibility to pay all the duties, taxes, and fees associated with importation and The Customs.
  5. It is important to get The Customs clearance before purchasing our products. We will not refund the purchase if the products are returned by The Customs.
  6. MSDS for each medium may be downloaded from this page under each product. MSDS may be needed for The Customs clearance.

Please email us at info@exptec.com for more information.

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